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| 10. |  | FEDERICI, M.; VALLEJOS, M.; SIMÓN, C.; ROVIRA, P.J.; CIGANDA, V.; RIGAMONTI, N.; FERRARI, G. Impacto de las cianotoxinas en la producción agropecuaria y el ambiente. Sustentabilidad. Revista INIA Uruguay, Marzo 2022, no.68, p.79-83 (Revista INIA; 68).| Biblioteca(s): INIA Las Brujas. |
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| 11. | | PACHECO-ROMERO, M.; VALLEJOS, M.; PARUELO, J.; ALCARAZ-SEGURA, D.; TORRES-GARCÍA, M. T.; SALINAS-BONILLO, M. J.; CABELLO, JAVIER A data-driven methodological routine to identify key indicators for social-ecological system archetype mapping. Environmental Research Letters, 2022, Volume 17, Issue 4, Article number 045019. OPEN ACCESS. doi: https://doi.org/10.1088/1748-9326/ac5ded Article history: Received 2 July 2021; Revised 28 January 2022; Accepted for publication 15 March 2022; Published 30 March 2022.
License: Original content from this work may be used under the terms of the Creative Commons Attribution 4.0...| Biblioteca(s): INIA Las Brujas. |
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| 13. | | VALLEJOS, M.; AGUIAR, S.; BALDI, G.; MASTRÁNGELO, M.E.; GALLEGO, F.; PACHECO-ROMERO, M; ALCARAZ-SEGURA, D.; PARUELO, J. Social-ecological functional types: connecting people and ecosystems in the Argentine Chaco. Ecosystems, 1 April 2020, Volume 23, Issue 3, Pages 471-484. Doi: https://doi.org/10.1007/s10021-019-00415-4 Article history: Received 10 January 2019// Accepted 21 June 2019//First Online 09 July 2019.| Biblioteca(s): INIA La Estanzuela. |
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| 14. | | AUER, A.; VON BELOW, J.; NAHUELHUAL, L.; MASTRANGELO, M.; GONZÁLEZ, A.; GLUCH, M.; VALLEJOS, M.; STAIANO, L.; LATERRA, P.; PARUELO, J. The role of social capital and collective actions in natural capital conservation and management. Environmental Science and Policy, May 2020, Volume 107, Pages 168-178. Doi: https://doi.org/10.1016/j.envsci.2020.02.024 Article history: Received 24 May 2019; Revised 21 December 2019; Accepted 27 February 2020; Available online 12 March 2020.
Corresponding author: Auer, A.; EEA Balcarce, Instituto Nacional de Tecnología Agropecuaria (INTA), Balcarce,...| Biblioteca(s): INIA Las Brujas. |
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| 15. | | AGUIAR, S.; MASTRANGELO, M.E.; GARCÍA COLLAZO, M.A.; CAMBA SANS, G.H.; MOSSO, C.E.; CIUFFOLI, L.; SCHMIDT, M.; VALLEJOS, M.; LANGBEHN, L.; CÁCERES, D.; MERLINSKY, G.; PARUELO, J.; SEGHEZZO, L.; STAIANO, L.; TEXEIRA, M.; VOLANTE, J.N.; VERÓN, S.R. Cuál es la situación de la Ley de Bosques en la Región Chaqueña a diez años de su sanción? Revisar su pasado para discutir su futuro. [What is the status of the forest law in the chaco region ten years after its enaction? Reviewing its past to discuss its future?]. Ecologia Austral, August 2018, vol.28, no.2, p. 400-417. OPEN ACCESS. EID: 2-s2.0-85052534872 Article history: Received 23 November 2016. / Accepted 6 March 2018.
Este trabajo fue financiado por el proyecto PICTo 2014-0046 "Bosques nativos, servicios ecosistémicos y bienestar humano en el Norte del Chaco Seco argentino: un...| Biblioteca(s): INIA Las Brujas. |
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| 16. | | ZARBÁ, L.; PIQUER-RODRÍGUEZ, M.; BOILLAT, S.; LEVERS, C.; GASPARRI, I.; AIDE, T. M.; ÁLVAREZ-BERRÍOS, N. L.; ANDERSON, L. O.; ARAOZ, E.; ARIMA, E.; BATISTELLA, M.; CALDERÓN-LOOR, M.; ECHEVERRÍA, C.; GONZALEZ-ROGLICH, M.; JOBBÁGY, E. G.; MATHEZ-STIEFEL, S.-L.; RAMIREZ-REYES, C-; PACHECHO, A.; VALLEJOS, M.; YOUNG, K. R.; GRAU, R. Mapping and characterizing social-ecological land systems of South America. Ecology and Society, 2022, Volume 27, Issue 2, Article number 27. OPEN ACCESS. doi: https://doi.org/10.5751/ES-13066-270227 Article: Gold Open Access, Green Open Access. -- Erratum: On 6 June 2022 the abstract was edited. See online for more detail: https://ecologyandsociety.org/vol27/iss2/art27/#dataarchive_stmt --
LICENSE: Published under license by The...| Biblioteca(s): INIA Las Brujas. |
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| 17. | | BAEZA, S.; VÉLEZ-MARTIN, E.; DE ABELLEYRA, D.; BANCHERO, S.; GALLEGO, F.; SCHIRMBECK, J.; VERON, S.; VALLEJOS, M.; WEBER, E.; OYARZABAL, M.; BARBIERI, A.; PETEK, M.; GUERRA LARA, M.; SARRAILHÉ, S.S.; BALDI, G.; BAGNATO, C.; BRUZZONE, L.; RAMOS, S.; HASENACK, H. Two decades of land cover mapping in the Río de la Plata grassland region: The MapBiomas Pampa initiative. Remote Sensing Applications: Society and Environment, 2022, Volume 28, Article 100834. doi: https://doi.org/10.1016/j.rsase.2022.100834 Article history: Received 26 April 2022; Received in revised form 29 August 2022; Accepted 3 September 2022; Available online 8 September 2022.
Corresponding author: Baeza, S.; Departamento de Sistemas Ambientales, Facultad de Agronomía,...| Biblioteca(s): INIA Las Brujas. |
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| 18. | | LEADLEY, P.; GONZALEZ, A.; OBURA, D.; KRUG, C.B.; LONDOÑO-MURCIA, M.C.; MILLETTE, K.L.; RADULOVICI, A.; RANKOVIC, A.; SHANNON, L.J.; ARCHER, E.; ATO ARMAH, F.; NIC BAX, N,; CHAUDHARI, K.; COSTELLO, M.J.; DÁVALOS, L.M.; ROQUE, F DE O; DECLERCK, F.; DEE, L.E.; ESSL, F.; FERRIER, S.; GENOVESI, P.; GUARIGUATA, M.R.; HASHIMOTO, S.; IFEJIKA SPERANZA, CH.; ISBELL, F.; KOK, M.; LAVERY, S.D.; LECLÈRE, D.; LOYOLA, R.; LWASA, S.; MCGEOCH, M.; MORI, A.S.; NICHOLSON, E.; OCHOA, J.M.; ÖLLERER, K.; POLASKY, S.; RONDININI, C.; SCHROER, S.; SELOMANE, O.; SHEN, X.; STRASSBURG, B.; RASHID SUMAILA, U.; TITTENSOR, D.P.; TURAK, E.; URBINA, L.; VALLEJOS, M.; VÁZQUEZ-DOMÍNGUEZ, E.; VERBURG, P.H.; VISCONTI, P.; WOODLEY, S.; XU, J. Achieving global biodiversity goals by 2050 requires urgent and integrated actions. One Earth, 2022, Volume 5, Issue 6, Pages 597-603. doi: https://doi.org/10.1016/j.oneear.2022.05.009 Artticle history: Available online 17 June 2022, Version of Record 17 June 2022.| Biblioteca(s): INIA La Estanzuela. |
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Registro completo
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Biblioteca (s) : |
INIA La Estanzuela; INIA Tacuarembó. |
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Fecha actual : |
01/12/2020 |
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Actualizado : |
21/05/2021 |
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Tipo de producción científica : |
Artículos en Revistas Indexadas Internacionales |
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Circulación / Nivel : |
Internacional - -- |
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Autor : |
PARODI, P.; CORBELLINI, L.G.; LEOTTI, V.B.; RIVERO, R.; MIRABALLES, C.; RIET-CORREA, F.; VENZAL, J.M.; ARMÚA-FERNÁNDEZ, M.T. |
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Afiliación : |
PABLO ANDRÉS PARODI TEXEIRA, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; LUIS G. CORBELLINI, Laboratório de Epidemiología Veterinária, Facultad de Veterinária, Departamento de Medicina Veterinária Preventiva, Universidade Federal do Rio Grande do Sul, Porto Alegre, Brazil; VANESSA B. LEOTTI, Departamento de Estatística, Instituto de Matemática e Estatística, Universidade Federal do Rio Grande do Sul, Porto Alegre, Brazil; RODOLFO RIVERO, Laboratorio Regional Noroeste “Miguel C. Rubino”, División de Laboratorios Veterinarios “Miguel C. Rubino”, Paysandú, Uruguay; MÓNICA CECILIA MIRABALLES FERRER, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; FRANKLIN RIET-CORREA AMARAL, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; JOSÉ M. VENZAL, Laboratorio de Vectores y enfermedades transmitidas, Facultad de Veterinaria, CENUR Litoral Norte, Universidad de la República, Salto, Uruguay; MARÍA T. ARMÚA-FERNÁNDEZ, Laboratorio de Vectores y enfermedades transmitidas, Facultad de Veterinaria, CENUR Litoral Norte, Universidad de la República, Salto, Uruguay. |
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Título : |
Validation of a multiplex PCR assay to detect Babesia spp. and Anaplasma marginale in cattle in Uruguay in the absence of a gold standard test. (Full Scientific Report) |
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Fecha de publicación : |
2021 |
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Fuente / Imprenta : |
Journal of Veterinary Diagnostic Investigation, Volume 33, Issue 1, January 2021, Pages 73-79. DOI: https://doi.org/10.1177/1040638720975742 |
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DOI : |
10.1177/1040638720975742 |
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Idioma : |
Inglés |
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Notas : |
Article history: Article first published online: November 30, 2020; Issue published: January 1, 2021. Acknowledgments: We thank Dra. María Angélica Solari (DILAVE-Montevideo), and the veterinarians and farmers who submitted samples. Corresponding author: Pablo Parodi: pabloparodi7@gmail.com |
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Contenido : |
Abstract: Detection of bovine Babesia spp. and Anaplasma marginale is based on the reading of Giemsa-stained blood or organ smears, which can have low sensitivity. Our aim was to improve the detection of bovine Babesia spp. and A. marginale by validating a multiplex PCR (mPCR). We used 466 samples of blood and/or organs of animals with signs and presumptive autopsy findings of babesiosis or anaplasmosis. The primers in our mPCR amplified the rap-1a gene region of Babesia bovis and B. bigemina, and the msp-5 region of A. marginale. We used a Bayesian model with a non-informative priori distribution for the prevalence estimate and informative priori distribution for estimation of sensitivity and specificity. The sensitivity and specificity for smear detection of Babesia spp. were 68.6% and 99.1%, and for A. marginale 85.6% and 98.8%, respectively. Sensitivity and specificity for mPCR detection for Babesia spp. were 94.2% and 97.1%, and for A. marginale 95.2% and 92.7%, respectively. Our mPCR had good accuracy in detecting Babesia spp. and A. marginale, and would be a reliable test for veterinarians to choose the correct treatment for each agent. |
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Palabras claves : |
ANAPLASMA MARGINALE; BABESIA SPP; CATTLE; ENFERMEDADES DE LOS ANIMALES; MULTIPLEX PCR; PLATAFORMA DE SALUD ANIMAL; SENSITIVITY; SPECIFICITY; TICK-BORNE DISEASES. |
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Asunto categoría : |
E16 Enfermedades de los animales |
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Marc : |
LEADER 02587naa a2200337 a 4500
001 1061695
005 2021-05-21
008 2021 bl uuuu u00u1 u #d
024 7 $a10.1177/1040638720975742$2DOI
100 1 $aPARODI, P.
245 $aValidation of a multiplex PCR assay to detect Babesia spp. and Anaplasma marginale in cattle in Uruguay in the absence of a gold standard test. (Full Scientific Report)$h[electronic resource]
260 $c2021
500 $aArticle history: Article first published online: November 30, 2020; Issue published: January 1, 2021. Acknowledgments: We thank Dra. María Angélica Solari (DILAVE-Montevideo), and the veterinarians and farmers who submitted samples. Corresponding author: Pablo Parodi: pabloparodi7@gmail.com
520 $aAbstract: Detection of bovine Babesia spp. and Anaplasma marginale is based on the reading of Giemsa-stained blood or organ smears, which can have low sensitivity. Our aim was to improve the detection of bovine Babesia spp. and A. marginale by validating a multiplex PCR (mPCR). We used 466 samples of blood and/or organs of animals with signs and presumptive autopsy findings of babesiosis or anaplasmosis. The primers in our mPCR amplified the rap-1a gene region of Babesia bovis and B. bigemina, and the msp-5 region of A. marginale. We used a Bayesian model with a non-informative priori distribution for the prevalence estimate and informative priori distribution for estimation of sensitivity and specificity. The sensitivity and specificity for smear detection of Babesia spp. were 68.6% and 99.1%, and for A. marginale 85.6% and 98.8%, respectively. Sensitivity and specificity for mPCR detection for Babesia spp. were 94.2% and 97.1%, and for A. marginale 95.2% and 92.7%, respectively. Our mPCR had good accuracy in detecting Babesia spp. and A. marginale, and would be a reliable test for veterinarians to choose the correct treatment for each agent.
653 $aANAPLASMA MARGINALE
653 $aBABESIA SPP
653 $aCATTLE
653 $aENFERMEDADES DE LOS ANIMALES
653 $aMULTIPLEX PCR
653 $aPLATAFORMA DE SALUD ANIMAL
653 $aSENSITIVITY
653 $aSPECIFICITY
653 $aTICK-BORNE DISEASES
700 1 $aCORBELLINI, L.G.
700 1 $aLEOTTI, V.B.
700 1 $aRIVERO, R.
700 1 $aMIRABALLES, C.
700 1 $aRIET-CORREA, F.
700 1 $aVENZAL, J.M.
700 1 $aARMÚA-FERNÁNDEZ, M.T.
773 $tJournal of Veterinary Diagnostic Investigation, Volume 33, Issue 1, January 2021, Pages 73-79. DOI: https://doi.org/10.1177/1040638720975742
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